Skip to main content

Gregory E. Crawford, PhD

Associate Professor in Pediatrics
Associate Professor in Molecular Genetics and Microbiology
Affiliate of the Regeneration Next Initiative
Campus mail: Box 3382 Med Ctr, Durham, NC 27710
Phone: (919) 684-8196
Email address: greg.crawford@duke.edu

My research involves identifying gene regulatory elements across the genome to help us understand how chromatin structure dictates cell function and fate. For the last 30 years, mapping DNase I hypersensitive sites has been the gold standard method to identify the location of active regulatory elements, including promoters, enhancers, silencers, and locus control regions. I have developed technologies that can identify most DNase I hypersensitive sites from potentially any cell type from any species with a sequenced genome. We are combining this data with other wet-lab and computational data types to better understand how these regulatory regions control global gene expression in a set of diverse tissues (normal and diseased) representative of the human body.

Education and Training

  • Postdoctoral Research Fellow, National Human Genome Research Institute, National Institutes of Health, 2001 - 2006
  • Ph.D., University of Michigan at Ann Arbor, 2001

Selected Grants and Awards

Publications

Crowley, James J., Jin Szatkiewicz, Anna K. Kähler, Paola Giusti-Rodriguez, NaEshia Ancalade, Jessica K. Booker, Jennifer L. Carr, et al. “Common-variant associations with fragile X syndrome..” Mol Psychiatry 24, no. 3 (March 2019): 338–44. https://doi.org/10.1038/s41380-018-0290-3.

Full Text

Coleman, Jonathan R. I., Julien Bryois, Héléna A. Gaspar, Philip R. Jansen, Jeanne E. Savage, Nathan Skene, Robert Plomin, et al. “Biological annotation of genetic loci associated with intelligence in a meta-analysis of 87,740 individuals..” Mol Psychiatry 24, no. 2 (February 2019): 182–97. https://doi.org/10.1038/s41380-018-0040-6.

Full Text

Barbu, M. C., Y. Zeng, X. Shen, S. R. Cox, T. K. Clarke, J. Gibson, M. J. Adams, et al. “Association of Whole-Genome and NETRIN1 Signaling Pathway–Derived Polygenic Risk Scores for Major Depressive Disorder and White Matter Microstructure in the UK Biobank.” Biological Psychiatry: Cognitive Neuroscience and Neuroimaging 4, no. 1 (January 1, 2019): 91–100. https://doi.org/10.1016/j.bpsc.2018.07.006.

Full Text

Wang, Daifeng, Shuang Liu, Jonathan Warrell, Hyejung Won, Xu Shi, Fabio C. P. Navarro, Declan Clarke, et al. “Comprehensive functional genomic resource and integrative model for the human brain..” Science 362, no. 6420 (December 14, 2018). https://doi.org/10.1126/science.aat8464.

Full Text

Amiri, Anahita, Gianfilippo Coppola, Soraya Scuderi, Feinan Wu, Tanmoy Roychowdhury, Fuchen Liu, Sirisha Pochareddy, et al. “Transcriptome and epigenome landscape of human cortical development modeled in organoids..” Science 362, no. 6420 (December 14, 2018). https://doi.org/10.1126/science.aat6720.

Full Text

Whitley, Sarah K., Anand Balasubramani, Carlene L. Zindl, Ranjan Sen, Yoichiro Shibata, Gregory E. Crawford, Nathaniel M. Weathington, Robin D. Hatton, and Casey T. Weaver. “IL-1R signaling promotes STAT3 and NF-κB factor recruitment to distal cis-regulatory elements that regulate Il17a/f transcription..” J Biol Chem 293, no. 41 (October 12, 2018): 15790–800. https://doi.org/10.1074/jbc.RA118.002721.

Full Text

Lee, Dongwon, Ashish Kapoor, Alexias Safi, Lingyun Song, Marc K. Halushka, Gregory E. Crawford, and Aravinda Chakravarti. “Human cardiac cis-regulatory elements, their cognate transcription factors, and regulatory DNA sequence variants..” Genome Res 28, no. 10 (October 2018): 1577–88. https://doi.org/10.1101/gr.234633.118.

Full Text

McDowell, Ian C., Alejandro Barrera, Anthony M. D’Ippolito, Christopher M. Vockley, Linda K. Hong, Sarah M. Leichter, Luke C. Bartelt, et al. “Glucocorticoid receptor recruits to enhancers and drives activation by motif-directed binding..” Genome Res 28, no. 9 (September 2018): 1272–84. https://doi.org/10.1101/gr.233346.117.

Full Text

D’Ippolito, Anthony M., Ian C. McDowell, Alejandro Barrera, Linda K. Hong, Sarah M. Leichter, Luke C. Bartelt, Christopher M. Vockley, et al. “Pre-established Chromatin Interactions Mediate the Genomic Response to Glucocorticoids..” Cell Syst 7, no. 2 (August 22, 2018): 146-160.e7. https://doi.org/10.1016/j.cels.2018.06.007.

Full Text

Bryois, Julien, Melanie E. Garrett, Lingyun Song, Alexias Safi, Paola Giusti-Rodriguez, Graham D. Johnson, Annie W. Shieh, et al. “Evaluation of chromatin accessibility in prefrontal cortex of individuals with schizophrenia..” Nat Commun 9, no. 1 (August 7, 2018). https://doi.org/10.1038/s41467-018-05379-y.

Full Text

Pages