The new Duke School of Medicine Cellular Metabolism Analysis Core Facility, housed in the Department of Pediatrics, features a Seahorse XFe96 Flux Analyzer by Agilent Technologies, Inc. The Seahorse Analyzer is an instrument used for studies of cellular energy metabolism. The instrument determines energy utilization in living cells in real-time, by measuring both oxygen consumption rate (OCR) and the change in pH/extracellular acidification rate (ECAR) to assess multiple cellular metabolic functions. XF Technology has been used with a variety of cell types including primary cells, adherent and suspension cell lines. Additionally, Agilent has developed assay kits and reagents to provide standard methods for quantifying mitochondrial respiration, glycolytic activity, endogenous and exogenous fatty acid oxidation, substrate oxidation, and metabolic switching. Cellular metabolism has a critical role in determining cell number and function. These cell metabolic and functional properties are oftentimes intimately related, such that disruption of normal cell metabolism can lead to altered cell biology. For these reasons, the Seahorse XFe96 Analyzer provides a key technology to measure cellular bioenergetics to yield information which will dramatically improve the ability of the investigators to determine the relevance of these metabolic readouts on cellular number and function in both health and disease.
The new core facility is managed by:
Nancie MacIver, MD, PhD
Associate Professor of Pediatrics (Endocrinology)
Assistant Professor of Immunology
Assistant Professor in Pharmacology and Cancer Biology
Member of the Duke Molecular Physiology Institute
Lab location: MSRB 1, Room 237
No training is needed, but an initial consultation is required. Cost is $230 per plate (maximum of 30 samples/plate). The cost includes consultation with Dr. MacIver and/or other experienced members of the lab, cost of the plate, media, all other reagents, running the instrument, and assistance with interpretation of results as needed.
Interested parties can email the core lab at firstname.lastname@example.org or contact Dr. MacIver directly.